Seleetive Incorporation f Polyunsaturated Animal Cells Fatty Acids into Organelle Phospholipids of Hironori MAsui , Reiko URADE
نویسندگان
چکیده
cells as described previously.2} Fctal calf serum (Walkerville, MD) was delipidated by tl'ic method described by Chani and Knowles.3) The cells were routine]y maintained in Eagle7$ minimum essential medium with 6va) de]jpidated fetal calf serum (medium A) and ineubated at 37"C gncler a 5V{i C02 atmosphcre at 1OO% rclative humidity, The cetls were seeded in the mediurn A at 1.8 x 104 cellsi'cm2. After 24h ef culture. the medium was replaced with fresh medium A containing 50uM UFA(s) complexed to fatty
منابع مشابه
Dietary Polyunsaturated Fatty Acids and Inflammation: The Role of Phospholipid Biosynthesis
The composition of fatty acids in the diets of both human and domestic animal species can regulate inflammation through the biosynthesis of potent lipid mediators. The substrates for lipid mediator biosynthesis are derived primarily from membrane phospholipids and reflect dietary fatty acid intake. Inflammation can be exacerbated with intake of certain dietary fatty acids, such as some ω-6 poly...
متن کاملCompetitive incorporation of dietary omega-3 and omega-6 polyunsaturated fatty acids into the tissue phospholipids in rats.
The incorporation of dietary omega-3 and omega-6 polyunsaturated fatty acids into the phospholipids of the liver, plasma and heart was studied by feeding rats with diets of increasing amounts of fish oil and/or corn oil up to 5% each for 2 weeks. Irrespective of the sources and amounts of dietary fat, total unsaturated fatty acids were 53-60% of phospholipid fatty acids in liver and plasma, and...
متن کاملSelective internalization of arachidonic acid by endothelial cells.
The asymmetric distribution of phospholipids in bovine endothelial-cell membranes was probed with 2,4,6-trinitrobenzenesulphonate and purified phospholipase A2. The data suggest that phosphotidylethanolamine is primarily located in the inner lipid bilayer, as reported for other cell types. Stearic acid is taken up by the endothelial cells and is randomly distributed among the membrane phospholi...
متن کاملPolyunsaturated fatty acids stimulate phosphatidylcholine synthesis in PC12 cells.
The synthesis of phospholipids in mammalian cells is regulated by the availability of three critical precursor pools: those of choline, cytidine triphosphate and diacylglycerol. Diacylglycerols containing polyunsaturated fatty acids (PUFAs) apparently are preferentially utilized for phosphatide synthesis. PUFAs are known to play an important role in the development and function of mammalian bra...
متن کاملIncorporation of fatty acids by concanavalin A-stimulated lymphocytes and the effect on fatty acid composition and membrane fluidity.
The fatty acid compositions of the neutral lipid and phospholipid fractions of rat lymph node lymphocytes were characterized. Stimulation of rat lymphocytes with the T-cell mitogen concanavalin A resulted in significant changes in the fatty acid composition of both neutral lipids and phospholipids (a decrease in the proportions of stearic, linoleic and arachidonic acids and an increase in the p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2018